enzyme linked immunospot elispot kits  (Cellular Technology Ltd)

Journal: NPJ Vaccines

Article Title: Polyvalent mRNA vaccine targeting outer surface protein C affords multi-strain protection against Lyme disease

doi: 10.1038/s41541-025-01326-3

Figure Lengend Snippet: A ELISpot determination of IFN-γ and IL-4 production by draining lymph node cells following 24 h stimulation with an overlapping OspC peptide pool represented by spot-forming units (SFU) per 10 5 lymph node cells. B Flow cytometry analysis of activation-induced markers (AIM) expression (CD25 + OX40+) as a percentage of CD4 + T cells in the dLNs collected 4 weeks after boost vaccination following 18 h stimulation with an overlapping OspC peptide pool. C Flow cytometry analysis of the percentage of CD4+ (top) and CD8+ (bottom) T cells producing IFN-γ, IL-2, or TNF-α. n = 5 per group, error bars represent standard deviation. Statistical significance was calculated using independent two-tailed t -tests. ns not significant, * p value <0.05, ** p value <0.01, *** p value <0.001.

Article Snippet: The ELISpot assay was performed using a Mouse IFN-γ/IL-4 Double-Color ELISPOT kit according to the manufacturer’s instructions (ImmunoSpot by Cellular Technology Limited, Shaker Heights, Ohio, USA).

Techniques: Enzyme-linked Immunospot, Flow Cytometry, Activation Assay, Expressing, Standard Deviation, Two Tailed Test

Journal: Vaccines

Article Title: Effect of Different Adjuvants on the Immunogenicity of a Recombinant Herpes Zoster Vaccine in Mice, Rats and Non-Human Primates

doi: 10.3390/vaccines13111124

Figure Lengend Snippet: The levels of humoral and cellular immune responses induced in mice by different adjuvants. The data in the figure are presented as the mean and standard deviation (SD). ( A , B ) The specific IgG titers of VZV-gE antigen in the serum of mice 28 days after the second immunization were detected by enzyme-linked immunosorbent assay. ( A ) shows the single adjuvant combination group; ( B ) shows the composite adjuvant combination group. ***: p < 0.001, **/##: p < 0.01, */#: p < 0.05 indicates statistical differences between the two groups, ‘*’ indicates statistical differences between the experimental group and the negative group, ‘#’ indicates statistical differences between the experimental group and the positive group. ( C , D ) After peptide library stimulation, the levels of IFN-γ, TNF-α, and IL-2 cytokines produced by spleen cells of mice 28 days after the second immunization were detected by flow cytometry. ( C ) shows the single adjuvant group; ( D ) shows the composite adjuvant group. ###/bbb: p < 0.001, **/##/bb: p < 0.01, */#/a/b: p < 0.05 indicate statistical differences between the experimental group and the positive group, ‘*’ indicates statistical differences between the experimental group and the positive group of cytokines IFN-γ, ‘#’ indicates TNF-α, ‘a’ indicates IL-2, ‘b’ indicates the total number of cytokines. ( E – G ) The levels of gE-specific cytokines obtained by spleen cells of mice were detected by ELISpot. ( E ) shows the single adjuvant group, ( F ) shows the composite adjuvant group, and ( G ) shows the PBS control group, the positive stimulation group, the C2 group, and C8 group. ***/###: p < 0.001, **: p < 0.01, *: p < 0.05 indicate statistical differences between the experimental group and the positive group, ‘*’ indicates statistical differences between the experimental group and the positive group of cytokines IFN-γ, and ‘a’ indicates the total number of cytokines.

Article Snippet: Splenocytes (2 × 10 5 cells/well) of immunized mice were seeded in 96-well plates for further analysis with enzyme-linked immunospot (ELISpot) assay kits (MabTech, Stockholm, Sweden, catalog number 3441-4APW-10 for IL-2 and 3321-4AST-10 for IFN-γ) according to the manufacturer’s protocol [ ].

Techniques: Standard Deviation, Enzyme-linked Immunosorbent Assay, Adjuvant, Produced, Flow Cytometry, Enzyme-linked Immunospot, Control

Journal: Vaccines

Article Title: Effect of Different Adjuvants on the Immunogenicity of a Recombinant Herpes Zoster Vaccine in Mice, Rats and Non-Human Primates

doi: 10.3390/vaccines13111124

Figure Lengend Snippet: ( A ) ELISA test results showing the titers of anti-gE protein antibodies in the serum of each group of SD rats. ( B ) ELISpot test results indicating the number of IFN-γ spots produced by spleen cells of each group of SD rats after stimulation with peptide libraries at different collection points. ****/####: p < 0.0001, ***/###: p < 0.001, **/##: p < 0.01, */#: p < 0.05 indicate statistical differences between the two groups, ’*’ indicates a statistical difference between the low-dose group and the negative control group, and ’#’ indicates a statistical difference between the high-dose group and the negative control group.

Article Snippet: Splenocytes (2 × 10 5 cells/well) of immunized mice were seeded in 96-well plates for further analysis with enzyme-linked immunospot (ELISpot) assay kits (MabTech, Stockholm, Sweden, catalog number 3441-4APW-10 for IL-2 and 3321-4AST-10 for IFN-γ) according to the manufacturer’s protocol [ ].

Techniques: Enzyme-linked Immunosorbent Assay, Enzyme-linked Immunospot, Produced, Negative Control

Journal: Vaccines

Article Title: Effect of Different Adjuvants on the Immunogenicity of a Recombinant Herpes Zoster Vaccine in Mice, Rats and Non-Human Primates

doi: 10.3390/vaccines13111124

Figure Lengend Snippet: ( A ) ELISA test results for the antibody titers against gE protein in the serum of each group of rhesus monkeys at different collection time points. ( B , C ) ELISpot test results for the number of IL-2 ( B ) and IFN-γ ( C ) spots produced by the spleen cells of each group of rhesus monkeys after stimulation with the peptide library. ****/####: p < 0.0001, **/##: p < 0.01, */#: p < 0.05 indicate statistical differences between the two groups, ’*’ indicates a statistical difference between the low-dose group and the negative control group, ‘#’ indicates a statistical difference between the high-dose group and the negative control group, and ‘a’ indicates a statistical difference between the high-dose group and the low-dose group.

Article Snippet: Splenocytes (2 × 10 5 cells/well) of immunized mice were seeded in 96-well plates for further analysis with enzyme-linked immunospot (ELISpot) assay kits (MabTech, Stockholm, Sweden, catalog number 3441-4APW-10 for IL-2 and 3321-4AST-10 for IFN-γ) according to the manufacturer’s protocol [ ].

Techniques: Enzyme-linked Immunosorbent Assay, Enzyme-linked Immunospot, Produced, Negative Control

Journal: Frontiers in Immunology

Article Title: Identification of immunogenic KIF5B-RET fusion neopeptides driving immune stimulation in tumor specific CD8+ T cells

doi: 10.3389/fimmu.2025.1635810

Figure Lengend Snippet: IFN-γ ELISpot of CD8+ T-Cells Stimulated with KIF5B-RET Neoantigenic Junction Peptides. NC: Non-Stimulated Control, NCP: Non-Activation Control Peptide, Pool: Neoantigen Junction Pool. T cell responses are considered positive if >20 spots/1M cells were counted, and the mean spot count was at least three-fold higher than the mean spot count of the NC. (*significantly positive T cell responses). D1/D2: Donor 1/Donor 2. Peptides colored in blue and red by representation of KIF5B or RET amino acids, respectively. Whiskers represent Standard Error of the Mean (SEM).

Article Snippet: To evaluate the peptide-stimulated CD8+ T cell immune response, IFN-γ production by cells stimulated with the predicted neoantigenic peptides was quantified using a commercially available Human IFN-γ ELISpot kit (CTL ImmunoSpot, Cellular Technology Ltd.), following the manufacturer’s instructions.

Techniques: Enzyme-linked Immunospot, Control, Activation Assay